LOCUS       40924_38463        7095 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plasmid vector pSAP2-g-multi DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Miichi F.
  TITLE     Construction of plasmid vector pSAP2-g-multi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Miichi F, Yoshida H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2017) Contact:Fumika Miichi Saga University, 
            Department of Biomolecular Sciences; 5-1-1 Nabeshima, Saga, Saga 
            849-8501, Japan
REFERENCE   3  (bases 1 to 7095)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7095)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JUN-2017) Contact:Fumika Miichi Saga University, Department of 
            Biomolecular Sciences; 5-1-1 Nabeshima, Saga, Saga 849-8501, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           659..1249
                     /gene="EhActin 5' region"
     gene            659..1249
                     /gene="EhActin 5' region"
                     /label=EhActin 5' region
     CDS             1250..2041
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     5'UTR           2051..3056
                     /gene="EhActin 3' region"
     gene            2051..3056
                     /gene="EhActin 3' region"
                     /label=EhActin 3' region
     rep_origin      3077..4363
                     /label=Autonomous replication sequence
                     /note="Autonomous replication sequence"
     5'UTR           4370..4843
                     /gene="EhAP-a 5' region"
     gene            4370..4843
                     /gene="EhAP-a 5' region"
                     /label=EhAP-a 5' region
     promoter        complement(4910..4928)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4949..4965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4973..4989)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4997..5027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5042..5063)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5351..5939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6113..6970)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6971..7075)
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacctc
      661 atggacaata ctctcctatt ttcttccaat ttttagattg tgttaatatt ctaatacatc
      721 aatttccaga tgattttgaa tttaacgaac aatttctttt agaattaaat gatgctatat
      781 tttgttctga ttttggaact ttcatgtttg actgtgaaaa gacaagaaaa gaaagtggtg
      841 ttgaacttca aacaccatca ttttgggaat atgttgttgg acagaaatgt aaatttatta
      901 atccaagtta taaagatgaa acagaagaat taatacttga agacaaacaa ccaaaattaa
      961 ttctttggac aaaatatttc acaagataca aaattaaaat gtcacgatga atttaaggtt
     1021 atacatcaca aataattaaa aagagatttt atatttgatt gttttaaggt tttaataatg
     1081 agaaaaataa aaagtcacct ctgaagaaaa ctgaaaatag gaaaaattcc aaaaatacaa
     1141 ggaaggaact tataaaataa cgtaaggaac tatgaagttc accttcagta aaaaagaaga
     1201 aagacactat ttaaagactg acaaaaactg aattgaacat tcaataaata tgattgaaca
     1261 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     1321 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     1381 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     1441 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     1501 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     1561 atctcacctt gctcctgccg agaaggtatc catcatggct gatgcaatgc ggcggctgca
     1621 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     1681 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgagg agcatcaggg
     1741 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     1801 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     1861 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     1921 tacccgtgat attgctgagg agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     1981 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     2041 ctgagtcgac gcgttttaat ttactttctc atttgataag ttttaaatta atgtttttaa
     2101 agaataagaa aataaaaaat ggtaataaga taaatttggt tttagatatt attttaatta
     2161 ttcaagagaa aataaatagt gttataaata acaaataata aaatagaatc aaaaaaagaa
     2221 aaaaaacaca aacctcaaat ataaaaaagt tcaatgaact gagaaaaaaa tagaaagaga
     2281 gtcaaaaaga gaaatgatga ataattacat acaatcatat tgcaaatatg gaagaattat
     2341 aagatatatt aatcttcttt ctaatggaac tattggttct caagaacttc ttgaagccat
     2401 tgattataca gaattaacta agaatattgg aggatttgca cgattaaaag aagcagtttc
     2461 atcaacactt ccattagatg aaccagaatg gaaaaaaaag ataggatatt cattatttga
     2521 acaagttaaa gcattagaaa cagaaataca aagtacatat ggagatgaag agagagctaa
     2581 attattatat aaattatcag acattcatga agaacatgga gaatatagta aagcaataaa
     2641 aaatgttctt caagcagttg agagtttaaa aaataaaatg atgtgtgctg aaggatatta
     2701 tcgattagtt agattaaata tatttaatga aaactttcat caagcaacta attttttaaa
     2761 taaattacaa tcattagatg catttacagg gaatttatat actaaattta ttaatattat
     2821 ttcattttta ttagcaatta gaaatactga aacatttata actgcatttg aattattaga
     2881 taaagttact acatttgatg aaaaagataa ttgggaattt ggtgagtttt taagttttca
     2941 agacattgct atttatggta cattaattgg attattaaca cagaaacatc aaacaaatgt
     3001 tattcattta attaatgatt caaaattcag aaatcatgca gacactgttc ctcgagccga
     3061 cggtatcgat aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc
     3121 accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     3181 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc
     3241 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct
     3301 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc
     3361 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat
     3421 cagtttttat actaatagat aggtataggg ataggtatag gtataggaaa aagagtataa
     3481 ataagtagaa ataagtatat agtcaagtac ataagcataa ataataaaga taataaagat
     3541 aataaagata ataataaaga taataataaa gataataata aagataataa taaagataat
     3601 aataaagata ataataaaga taataataaa gataataata atataaagga atagaaacat
     3661 atataaatat acataagtcg tggtaaaaga gaagtaaagt ataataagta taataagtat
     3721 aataagtata ataagtataa taagtataat aagtataata agtataataa gtataataag
     3781 tataataagt ataataagga atataaagaa taaagtctaa ggggcatata gtcatggtaa
     3841 atccattgtg gtgtagttgg taacatacaa gcctttcacg tttgtgcccc gggttcgatt
     3901 cccggcgatg gaatttttta gcaagaatat atatatatag tacacataag tacactacac
     3961 atatagtaca ctacacatat acataaaata aatatatact acacataata taaaatatat
     4021 acataatata aaatatatac ataatataaa gataagggat acataatata aatataaata
     4081 taaatatata cataatataa agataaggta tagagaaata acagggaaga gataataaga
     4141 agtatataaa gaataggtat aaagaataga aacatatata agcacatata gaaatataaa
     4201 aggatataaa aagaataaga aaagaataag aaaaggatat caaaagaata acaaaagaat
     4261 ataaaaaggt gatatcgata ccgaccatag ctcagttggt agagcggagg actgtagatg
     4321 tagaatagaa ttccttaggt cactggttcg aatccggttg gtcggatccc ttgctgcacc
     4381 ctttgcagct tccctaatac attcctaatt caaaggcacc gtcataacca gataataact
     4441 gggagagtcg aagtatgaat ttgtactagg gtttgtgtgt ggtgtttcag acgtgccacc
     4501 ttcgatcttt tattatttaa aaaacaaaat agaaaaaaat ataaaacact caaatgaaaa
     4561 aaaaaactta tgtcatatat agaatgttct tattctttaa gtattttaaa ttttttaata
     4621 aatattaaaa gagaagaaat gaaataatca atttaattat ttctgtttaa aaaaaaaagt
     4681 ttagaaaatt aaaaaaaaag aaaaagagaa gaaaaacgaa gatctaaaat ttgataaaaa
     4741 gcgtgactta aggaattcag ttttggtaga aaattcagcc acatcaccgt gaattattta
     4801 aagagtaggt tgaaaactac aaaaacatat cttacaaaca atcatgaggc ctccgcggac
     4861 tagttctaga gcggccgcca cccctttaaa cgagctccag cttttgttcc ctttagtgag
     4921 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5281 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5341 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5401 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5461 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5521 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5581 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5641 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5701 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5761 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     5821 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5881 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5941 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6001 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6061 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7081 ttccccgaaa agtgc
//
